Allows customized workflows for labs of all sizes promega this week introduced its new modular nucleic acid preparation system that allows labs of all sizes to adapt to changing workflows. Dys437, dys438 and dys439 with the powerplex y kit promega. The powerplex fusion system enables increased data sharing and greater discriminatory power than seen in previously available str systems. The powerplex y23 system also is compatible with direct amplification, enabling streamlined ystr databasing efforts. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. The powerplex fusion 6c system is a 6color, 27locus multiplex for human identification applications including forensic analysis, relationship testing and research use. Use of the powerplexr y23 system to amplify extracted dna. Promega introduces powerplex fusion 6c system for mixture. Prepare a loading cocktail by combining and mixing wen ils 500 y23 and formamide. A total of 96 different haplotypes were observed and the haplotype diversity was 0. The kit was designed to combine 21 autosomal str loci 1, 12. Promega new powerplexr y23 str system reveals more y. Median joining mj networks for ystr haplotypes within specific y haplogroups were.
Procedures manual and contact the fbis codis unit for further information. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Note that smallersized panels are included in the next larger panels. Promega is seeking united states ndis approval for. The powerplex 21 system is a very easy to use str system that gives the highest degree of confidence in sample calls. Promega corporation promega maxwell rsc viral total nucleic acid purification kit, 48 preps the maxwell rsc viral total nucleic acid purification kit is designed for automated extraction of viral total nucleic acid rna and dna from serum, plasma or other samples using the maxwell rsc instrument. Maxwell, plexor, powerplex and powerquant are registered trademarks of promega. Promega powerplex y23 catalog numbers dc2305dc2320. Overview of the powerplex y23 system includes all of the loci from powerplex y as well as ampflstr yfiler includes six ystr loci that are not in any other commercially available kit one system capable of extracted and direct amplification applications amplify male genomic dna in an excess of female genomic dna no amplification products for female genomic dna. In contrast, the 27plex powerplex fusion 6c system.
This component should be moved to the postamplification box after opening. Wsp cld forensic dna continuing education day june 18, 2015. The prototype powerplex y23 system promega corpora tion, madison, wi is a. We use the system to check the lineage of cell lines prior to the start of experiments or publications to assure that the lines we are working with are correct and have not mutated saving us the expense of rerunning the experiments. Developmental validation efforts are already under way. Demonstrate ability to combine str primers from 18plex with primers for. For each of these six panels differentsized databases exist. The purpose of the presentstudy is to validatethe powerplex1 y23 system using half volumereaction mix for the ampli. The system allows coamplification and fluorescent detection of 20 str loci and amelogenin.
Currently, the process to generate these profiles is typically conducted in 810 h using traditional laboratory methods. Genemarker hid str human identity software promega. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516 3. The kit contains all 12 loci in powerplex y system, the additional 5 loci found in ampf l str yfiler, plus 6 new loci. Developmental validation of the powerplex r y23 system. Fast, accurate and userfriendly 1documented time savings of up to 40% up to 70% less analyst intervention2 broad kit compatibility. The system combines common and informative loci to overcome typical challenges faced by laboratories, including sample inhibitors, low. Once the network settings are properly configured, you should be able to connect to the duet board and access the duet web console. Validation and implementation of the promega powerplex. The powerplex y23 system allows ystr analysis of human forensic and database samples. Learn how to interpret results from ystr dna typing using the ychromosome haplotype reference database yhrd, a free resource. Promega offers a range of products for cell biology and drug discovery, many of which are built upon bioluminescence technology. Prepare the pcr amplification mix by combining the components as shown below. A comprehensive portrait of ystr diversity of indian populations.
To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Choosing a kit started validation in mid2012 something we could drop into available workflow 5 dye chemistry, 3. Protocols for dna amplification using the powerplex y23 system. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. Promega was an early supplier in the cellfree protein synthesis field and is continuing to develop its portfolio in this area. Our teams are in regular contact with suppliers and distributors worldwide to manage inventory of raw materials to ensure continued availability. A protocol to amplify the loci d3s58, th01, d21s11, d18s51, penta e, d5s818, ds317, d7s820, d15s539, csf1po, penta d, vwa, d8s1179, tpox, fga and se33. The powerplex fusion 6c system is configured so that both casework and databasing sections of a laboratory purchase the same kit, simplifying validation and qc efforts. Use with extracted dna samples, punches and pretreated swabs.
Request pdf developmental validation of the powerplex r y23 system. You can connect your promega to your local network via the ethernet port on the duet maestro. Powerplex y23 system is a 23loci, 5color ystr multiplex designed for genotyping of database, paternity and forensic casework samples. M3d has commissioned the dream printer a featurepacked, expandable, industrial 3d printer designed for manufacturing, assembly and design operations. The system allows the coamplification and threecolor detection of sixteen loci fifteen str loci and amelogenin.
Internal validation and implementation of the powerplex. Powerplex y23 and mitochondrial control region in a. Promega has launched the powerplex fusion system, a 24locus multiplex assay that incorporates common and informative loci used throughout the world the powerplex fusion system enables increased data sharing and greater discriminatory power than. Powerplex y23 system reproducibly amplifies trace template amounts as. Email promega technical services if you have questions on use of this system. The promega is reliable, fast and overengineered to ensure high quality printing.